IB Internal Assessment (IA) Biology Standard level (SL) 2026 May Solution & Worked Answer

Investigating the Effect of Aloe Vera Concentration on the Rate of Wound Closure in Allium cepa Epidermal Cells

Solution Overview

This investigation explores how varying concentrations of aloe vera gel (0%–100%) affect the rate of wound closure in Allium cepa epidermal cells over a 24-hour period.

The methodology involves mechanical wounding, microscopic imaging of stained cells, and quantitative area measurement via grid calibration. Statistical analysis includes one-way ANOVA, Tukey post-hoc tests, and effect size (η²) calculations.

Findings reveal a significant dose-dependent relationship, where increased aloe vera concentrations accelerate the rate of wound healing. This exemplar is highly valuable for its rigorous statistical treatment, detailed uncertainty propagation, and a comprehensive evaluation of experimental limitations and improvements.

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