IB Internal Assessment (IA) Biology Standard level (SL) 2026 May Solution & Worked Answer

Investigating the Effect of Temperature on the Rate of Catalase Activity in Solanum tuberosum Tissue

Solution Overview

This investigation explores how temperature (25.0°C to 45.0°C) affects the rate of catalase activity in Solanum tuberosum tissue, measured via the height of oxygen foam produced from 5.0% hydrogen peroxide decomposition.

The methodology employs a controlled lab experiment with five replicates per temperature level. Data analysis includes mean calculations, standard deviation, and a One-Way ANOVA to determine statistical significance.

Findings identify an optimal temperature of approximately 35.0°C, with activity declining thereafter due to enzyme denaturation. The paper is distinguished by its rigorous inferential statistical analysis and a detailed evaluation of systematic and random errors, suggesting improvements like using gas syringes for direct oxygen measurement.

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