Investigating the Concentration of Amylase in Vigna radiata, Musa, and Honey using Starch-Agar Inhibition Zones

This investigation explores the difference in amylase concentrations among Vigna radiata, Musa, and honey. The student employed a primary lab experiment using starch-agar plates to measure inhibition zones, establishing a standard curve through cubic regression to interpolate unknown concentrations.

The study found that Vigna radiata possessed the highest amylase activity, with results validated by a One-Way ANOVA and post-hoc Tukey HSD test (p < 0.01). This exemplar is particularly valuable for its rigorous statistical approach, including detailed uncertainty propagation and a high coefficient of determination (R² ≈ 1.00), demonstrating a sophisticated method for quantifying enzyme activity in natural substrates.